A new microscope can capture real-time 3D videos of what’s happening inside cells down to the molecular level—feat once thought impossible.
https://www.meneame.net/story/nuevo-microscopio-hace-zoom-dentro-imagenes-3d-tiempo-real-ing
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Maximum intensity projections and measured GFP-EB1 trajectories for a single HeLa cell in metaphase. Growing microtubule endpoints and tracks are color coded by growth phase lifetime. Bounding box, 29 x 36 x 26 μm.
Credit: Betzig Lab, HHMI/Janelia Research Campus, Mimori-Kiyosue Lab, RIKEN Center for Developmental Biology; 10/24/14 issue of the journal Science.
For more information, see http://www.hhmi.org/news/new-microscope-collects-dynamic-images-molecules-animate-life
Various views of dorsal closure in a D. melanogaster embryo expressing sGMCA, a marker of filamentous actin at the plasma membrane, over 639 time points at 12.0 sec intervals. Top left: orthoslice through the center of the amnioserosa. Top right: maximum intensity projection through a thin rectangular slab encompassing the apical surface of the amnioserosa. Bottom right: MIP through a similar slab encompassing the basal surface of the amnioserosa. Bottom left, volume rendering, with the apical surface in blue/green and the basal surface in orange/red (cf., Fig. 6E, F). Bounding box, 73 x 73 x 26 μm.
Credit: Betzig Lab, HHMI/Janelia Research Campus, Kiehart Lab, Duke University; 10/24/14 issue of the journal Science.
For more information, see http://www.hhmi.org/news/new-microscope-collects-dynamic-images-molecules-animate-life
Volume rendering of the amnioserosa and the encroaching epithelium during dorsal closure in a D. melanogaster embryo expressing GFP/DE-cadherin to highlight cell-cell junctions, over 840 time points at 8 sec intervals (cf., Fig. 6C). Bounding box, 86 x 80 x 31 μm.
Credit: Betzig Lab, HHMI/Janelia Research Campus, Kiehart Lab, Duke University; 10/24/14 issue of the journal Science.
For more information, see http://www.hhmi.org/news/new-microscope-collects-dynamic-images-molecules-animate-life
Movie 5 High Resolution from HHMI NEWS on Vimeo.
